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The tissue samples were centrifuged at 2583 x g for 15 minutes. Quantitative analysis by HPLC For MBZ assay, cpphs 1208-1 binary. 5 ml plasma, cyst wall, cyst fluid and mices tissue samples with Binare optionen handelsgesellschaften as internal standard was extracted with Oasis HLB Cartridges (Waters, USA).

The final collection of methanol elution was dried and redissolved in methanol. The MBZ concentration was measured by cpphs 1208-1 binary absorbance at a wavelength of 289 nm.

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